Baylor College of Medicine, Department of Pediatrics, Immunology, Allergy and Retrovirology Service at Texas Children’s Hospital and the William T. Shearer Center for Human Immunobiology Forbes-Satter lab is seeking a highly motivated, responsible, and organized individual to join a research team focused on Immune dysregulation in the JAK/STAT signaling pathway. The Forbes-Satter lab is a rapidly growing program and the Research Technician II will help meet the needs of the program that serves the children and families of Houston and the surrounding areas, the Southeast Region of Texas, nationally, and internationally with rare immunologic disease.
Perform the routine elements of research work that requires the use of skills, technical and academic knowledge to follow established procedures and record results.
Interact with the supervisor on a daily basis, but will be expected to independently perform and execute successful experiments.
Coordinate communication between investigators and other members of the team and collaborators.
Work with a team to coordinate the collection, processing, and shipping of samples.
Handle blood and tissue samples with sterile technique.
Prepare and conduct laboratory research experiments.
Preparation of laboratory reagents and growth media.
Growth of E. coli bacterial cultures (all strains are non-pathogenic and completely safe).
Various molecular biology methods including, PCR, qPCR, and gel electrophoresis.
Molecular cloning and DNA analyses (recombinant DNA, PCR, sequencing).
Preparation of sequencing reactions, including multi-channel pippetting.
Transient and stable transfections.
Protein expression, purification, and analyses.
Flow cytometry of engineered cells.
Keeping a detailed account of all activity and results in a laboratory notebook.
Document results of experiments.
Order laboratory supplies and equipment.
Data Analysis: simple data analysis in excel spreadsheet format by performing arithmetical operations (multiplication, division, etc) on a set of data points, as well as obtaining the mean.
Conduct literature searches and summarizes information in an appropriate format for a particular study or project.
Select, evaluate and apply techniques used in routine research.
Maintain a clean and safe work environment.
Stock and order supplies.
Bachelor's degree in a Basic Science or related field. Four years of related experience may substitute for degree requirement.
No experience required.
Basic mathematical skills applicable to dilutions, suspensions, and concentration calculation.
Must have excellent pipetting skills.
Work with computer programs to analyze data results.
Familiarity with flow cytometry.
Must have excellent problem solving skills.
Must be willing to commute occasionally between Main Baylor and the Feigin Center.
Must be willing to learn new laboratory techniques and skills related to the project.
Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.
Baylor College of Medicine fosters diversity among its students, trainees, faculty and staff as a prerequisite to accomplishing our institutional mission, and setting standards for excellence in training healthcare providers and biomedical scientists, promoting scientific innovation, and providing patient-centered care.
- Diversity, respect, and inclusiveness create an environment that is conducive to academic excellence, and strengthens our institution by increasing talent, encouraging creativity, and ensuring a broader perspective.
- Diversity helps position Baylor to reduce disparities in health and healthcare access and to better address the needs of the community we serve.
- Baylor is committed to recruiting and retaining outstanding students, trainees, faculty and staff from diverse backgrounds by providing a welcoming, supportive learning environment for all members of the Baylor community.