Research Technologist 2

Northwestern University

Chicago, IL

Job posting number: #7070368

Posted: September 28, 2020

Application Deadline: Open Until Filled

Job Description

Job Summary:

The Research Technologist 2 conducts experimental research lab work and/or assists in design of one or more specific phase(s) of entire project(s). May complete mathematical calculations, and/or computer tasks and/or assist in judging validity of data. Follows appropriate protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices.

Specific Responsibilities:

Following protocols, designs, sets-up and conducts basic experiments.
Analyzes and troubleshoots protocols and data obtained from individual experiments and recommends alternatives and suggestions to improve processes, validity and/or accuracy.
Using a fully functioning level of skill, completes the following activities
Molecular Biology:

DNA, RNA, Protein isolation, purification and expression
Southern, Northern, Western blotting
PCR, RT-PCR
Plasmid preparation
Cloning, mapping and sequencing of DNA
Recombinant virus production and purification
Cellular Biology:

Cell culture, cell isolation
Functional studies
Transfection
Histology:

Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
Immunohistochemistry
Microscopy
Immunology:

Flow cytometry
ELISA
Animal:

Husbandry
Breeding and screening transgenic mice
Dissections
Blood collection
Surgical procedures
Database management of colony
Other (please specify):

Research Experience with mouse brain and muscle or stem cells a plus
Administration:

Maintains detailed records of results which may include collecting, extracting & entering data.
Manipulates, interprets &/or presents results.
Prepares charts & graphs for presentations & publications.
Performs scientific literature searches in support of research.
Completes portions of grant applications, manuscripts & publications.
Finance:

Tracks & summarizes lab supply expenses.
Verifies accuracy of invoices.
Orders &/or maintains inventory of reagent, mice &/or lab supplies.
Supervises:

Trains students.
Performs other related duties as assigned.



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