Dr. Joanna Yi's laboratory at Baylor College of Medicine (BCM)/Texas Children's Hospital is seeking a talented research technician III to work on developing targeted drugs for pediatric cancers in collaboration with BCM's Center for Drug Discovery (CDD). The Yi lab seeks to identify and drug relevant, lynchpin targets in pediatric cancers through a multidisciplinary approach including chemical biology, chromatin biology, cell and molecular biology, and assay development. Concurrently, we want to generate biomarker assays of activity that potentially could be used in clinical trials. We have unique access to screen our targets with the CDD's drug libraries, including the >3.1 billion DNA-encoded chemical library and the robotics available to design and run high-throughput screens. There is also a significant effort in the lab to characterize the transcriptional control of pediatric AML which should uncover novel, druggable dependencies in vitro and in vivo which will require validation before drug screening with the CDD.
Under supervision, will work on genetically validating an identified druggable target in pediatric AML (e.g. shRNA, CRISPR, etc) and also bringing the validated target to and through drug screening, including possibly protein purification but also biochemical assay development
Kinase and phosphatase assays
Cell cycle analysis
Biochemical and cell-based assay development (including use of drug and liquid handling devices)
Handling primary patient samples, including ex vivo drug treatments
Prepares and conducts laboratory research experiments
Collects, compiles and analyzes data
Documents results of experiments
Selects, evaluates and applies techniques used in routine research
Education Required: Bachelor's degree in a Basic Science or related field. Four years of related experience may substitute for degree requirement.
Experience Required: One year of relevant experience.
Master's degree in a related field.
Prefer at least one year of relevant experience in areas of cancer biology, molecular biology, chemical biology, or chromatin biology.
Experience with chromatin immunoprecipitation, assay development (cell-free and cell-based assays), compound management and drug combination experience, and recombinant protein production in bacterial and/or mammalian expression systems.
Should be highly motivated and ambitious, organized and anticipate problems, eager to take ownership of projects and productive in data generation, and willing to work with collaborators in a team.
Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.
Baylor College of Medicine fosters diversity among its students, trainees, faculty and staff as a prerequisite to accomplishing our institutional mission, and setting standards for excellence in training healthcare providers and biomedical scientists, promoting scientific innovation, and providing patient-centered care.
- Diversity, respect, and inclusiveness create an environment that is conducive to academic excellence, and strengthens our institution by increasing talent, encouraging creativity, and ensuring a broader perspective.
- Diversity helps position Baylor to reduce disparities in health and healthcare access and to better address the needs of the community we serve.
- Baylor is committed to recruiting and retaining outstanding students, trainees, faculty and staff from diverse backgrounds by providing a welcoming, supportive learning environment for all members of the Baylor community.